1.IMMUNIZATION
We immunize llamas with the molecule of interest, using different delivery methods depending on its nature.
2.WHITE BLOOD CELL ISOLATION
Next, we extract a small amount of blood from the immunized llama and isolate the white blood cells containing the antibody-producing cells.
3.NANOBODY CLONING
We extract cDNA from the white blood cells and amplify the entire repertoire of nanobodies generated during immunization. These nanobodies are cloned into appropriate vectors, resulting in a library with over 100 MILLION ANTIBODIES.
4.selecTION
We then select the best nanobodies for our specific application and genetically engineer them to be expressed in simple prokaryotic production systems.
